HSP47 Monoclonal antibody
HSP47 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A8F6
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HEK-293T cells, NIH/3T3 cells, LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells |
Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67863-1-Ig targets HSP47 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | rat |
Immunogen | HSP47 fusion protein Ag30999 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) |
Synonyms | 47 kDa heat shock protein, AsTP3, CBP1, CBP2, Collagen binding protein, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2 |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | BC014623 |
Gene Symbol | SERPINH1 |
Gene ID (NCBI) | 871 |
RRID | AB_2918621 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P50454 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HSP47 antibody 67863-1-Ig | Download protocol |
IHC protocol for HSP47 antibody 67863-1-Ig | Download protocol |
IF protocol for HSP47 antibody 67863-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Proteome Res Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts |