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HSP47/SERPINH1 Monoclonal antibody

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

2A8F6

Cat no : 67863-1-Ig

Print datasheet

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

验证数据展示

  • WB analysis using 67863-1-Ig

    WB analysis using 67863-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67863-1-Ig

    WB analysis of NIH/3T3 using 67863-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67863-1-Ig

    WB analysis using 67863-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 67863-1-Ig

    IHC staining of human renal cell carcinoma using 67863-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human renal cell carcinoma using 67863-1-Ig

    IHC staining of human renal cell carcinoma using 67863-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IF Staining of HeLa using 67863-1-Ig

    IF Staining of HeLa using 67863-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 67863-1-Ig

    IF Staining of HepG2 using 67863-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, HEK-293T cells, NIH/3T3 cells, LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67863-1-Ig targets HSP47/SERPINH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen HSP47/SERPINH1 fusion protein Ag30999 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms HSP47, SERPINH1, CBP2, CBP1, AsTP3
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC014623
Gene Symbol HSP47
Gene ID (NCBI) 871
RRIDAB_2918621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50454
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
IHC protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
IF protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

J Proteome Res

Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts

Authors - Bridgette Hartley
View Article
humanWB,IP,CoIP

Elife

Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum

Authors - Ya-Juan Wang
View Article