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CoraLite®594-conjugated HSP47 Monoclonal antibody

HSP47 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A8F6

Cat no : CL594-67863

Print datasheet

Synonyms

SERPINH1, CBP2, CBP1, AsTP3, Arsenic-transactivated protein 3

验证数据展示

  • IF Staining of HeLa using CL594-67863

    IF Staining of HeLa using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL594-67863

    IF Staining of HepG2 using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-67863

    FC experiment of HepG2 using CL594-67863

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human HSP47 (CL594-67863, Clone:2A8F6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67863-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67863 targets HSP47 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen HSP47 fusion protein Ag30999 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms SERPINH1, CBP2, CBP1, AsTP3, Arsenic-transactivated protein 3
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC014623
Gene Symbol HSP47
Gene ID (NCBI) 871
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50454
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 HSP47 antibody CL594-67863Download protocol
Standard Protocols
Click here to view our Standard Protocols