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验证数据展示

  • WB analysis using 67863-1-Ig

    WB analysis using 67863-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67863-1-Ig

    WB analysis of NIH/3T3 using 67863-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67863-1-Ig

    WB analysis using 67863-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 67863-1-Ig

    IHC staining of human renal cell carcinoma using 67863-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human renal cell carcinoma using 67863-1-Ig

    IHC staining of human renal cell carcinoma using 67863-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IF Staining of HeLa using 67863-1-Ig

    IF Staining of HeLa using 67863-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 67863-1-Ig

    IF Staining of HepG2 using 67863-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

HSP47/SERPINH1 Monoclonal antibody

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67863-1-Ig

产品说明书

CloneNo. 2A8F6
引用文献(3)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (3)

别名

HSP47, SERPINH1, CBP2, CBP1, AsTP3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293T cells, NIH/3T3 cells, LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67863-1-Ig targets HSP47/SERPINH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 HSP47/SERPINH1 fusion protein Ag30999 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
别名 HSP47, SERPINH1, CBP2, CBP1, AsTP3
计算分子量 46 kDa
观测分子量46 kDa
GenBank蛋白编号BC014623
基因名称 HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_2918621
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50454
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
IHC protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
IF protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

iScience

SERPINH1 modulates apoptosis by inhibiting P62 ubiquitination degradation to promote bone metastasis of prostate cancer

Authors - Chen Tang
  • KD Validated
View Article
ratWB

J Proteome Res

Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts

Authors - Bridgette Hartley
View Article
humanWB,IP,CoIP

Elife

Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum

Authors - Ya-Juan Wang
View Article