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HSP47 Monoclonal antibody, PBS Only

HSP47 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2A8F6

Cat No : 67863-1-PBS

Print datasheet

Synonyms

47 kDa heat shock protein, AsTP3, CBP1, CBP2, Collagen binding protein, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2

验证数据展示

  • WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

    WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP47 antibody included
  • IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

    IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

    IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.

产品信息

67863-1-PBS targets HSP47 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen HSP47 fusion protein Ag30999 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms 47 kDa heat shock protein, AsTP3, CBP1, CBP2, Collagen binding protein, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC014623
Gene Symbol SERPINH1
Gene ID (NCBI) 871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50454
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.