• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HSP47/SERPINH1 Monoclonal antibody, PBS Only (Detector)

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A8F6

Cat no : 67863-1-PBS

Print datasheet

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

验证数据展示

  • WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

    WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP47 antibody included
  • IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

    IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

    IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50781-1

    Cytometric bead array standard curve of MP50781-1

    Cytometric bead array standard curve of MP50781-1, HSP47/SERPINH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67863-2-PBS. Detection antibody: 67863-1-PBS. Standard:Ag30999. Range: 0.781-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.

产品信息

67863-1-PBS targets HSP47/SERPINH1 as part of a matched antibody pair:

MP50781-1: 67863-2-PBS capture and 67863-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HSP47/SERPINH1 fusion protein Ag30999 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms HSP47, SERPINH1, CBP2, CBP1, AsTP3
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC014623
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50454
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C