HSPB3 Monoclonal antibody
HSPB3 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rabbit, mouse, rat
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2A10B5
验证数据展示
经过测试的应用
Positive WB detected in | human heart tissue, mouse heart tissue, rabbit heart tissue, rat heart tissue |
Positive IHC detected in | rat kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67890-1-Ig targets HSPB3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rabbit, mouse, rat samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Tested Reactivity | Human, rabbit, mouse, rat |
Immunogen | HSPB3 fusion protein Ag26940 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | heat shock 27kDa protein 3 |
Synonyms | Heat shock 17 kDa protein, heat shock 27kDa protein 3, Heat shock protein beta 3, HSP 17, HSPB3, HSPL27 |
Calculated Molecular Weight | 150 aa, 17 kDa |
Observed Molecular Weight | 20 kDa |
GenBank Accession Number | BC126272 |
Gene Symbol | HSPB3 |
Gene ID (NCBI) | 8988 |
RRID | AB_2918646 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q12988 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HSPB3 antibody 67890-1-Ig | Download protocol |
IHC protocol for HSPB3 antibody 67890-1-Ig | Download protocol |
IF protocol for HSPB3 antibody 67890-1-Ig | Download protocol |
FC protocol for HSPB3 antibody 67890-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |