• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HeLa using CL594-67890

    FC experiment of HeLa using CL594-67890

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human HSPB3 (CL594-67890, Clone:2A10B5) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated HSPB3 Monoclonal antibody

HSPB3 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67890

产品说明书

CloneNo. 2A10B5

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rabbit, mouse, rat

应用

FC (Intra)

别名

HSPB3, HSPL27, Protein 3, 2A10B5, Heat shock protein beta-3, Heat shock protein family B member 3, HSP 17, HSP27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67890-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67890 targets HSPB3 in FC (Intra) applications and shows reactivity with Human, rabbit, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rabbit, mouse, rat
免疫原 HSPB3 fusion protein Ag26940 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 27kDa protein 3
别名 HSPB3, HSPL27, Protein 3, 2A10B5, Heat shock protein beta-3, Heat shock protein family B member 3, HSP 17, HSP27
计算分子量 150 aa, 17 kDa
观测分子量20 kDa
GenBank蛋白编号BC126272
基因名称 HSPB3
Gene ID (NCBI) 8988
RRIDAB_2923930
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12988
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 HSPB3 antibody CL594-67890Download protocol
Standard Protocols
Click here to view our Standard Protocols