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验证数据展示

  • WB analysis using 67890-1-Ig

    WB analysis using 67890-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of human heart using 67890-1-Ig

    WB analysis of human heart using 67890-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 67890-1-Ig

    IHC staining of mouse kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 67890-1-Ig

    IHC staining of mouse kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 67890-1-Ig

    IHC staining of mouse kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 67890-1-Ig

    IHC staining of rat kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 67890-1-Ig

    IHC staining of rat kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67890-1-Ig

    IF Staining of HeLa using 67890-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67890-1-Ig

    IF Staining of HeLa using 67890-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 67890-1-Ig

    FC experiment of HeLa using 67890-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human HSPB3 (67890-1-Ig, Clone:2A10B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HSPB3 Monoclonal antibody

HSPB3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67890-1-Ig

产品说明书

CloneNo. 2A10B5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

HSPL27, HSP27, HSP 17, Heat shock protein family B member 3, Heat shock protein beta-3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, mouse heart tissue, rabbit heart tissue, rat heart tissue
Positive IHC detected inrat kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67890-1-Ig targets HSPB3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 HSPB3 fusion protein Ag26940 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 27kDa protein 3
别名 HSPL27, HSP27, HSP 17, Heat shock protein family B member 3, Heat shock protein beta-3
计算分子量 150 aa, 17 kDa
观测分子量20 kDa
GenBank蛋白编号BC126272
基因名称 HSPB3
Gene ID (NCBI) 8988
RRIDAB_2918646
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12988
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.

实验方案

Product Specific Protocols
WB protocol for HSPB3 antibody 67890-1-IgDownload protocol
IHC protocol for HSPB3 antibody 67890-1-IgDownload protocol
IF protocol for HSPB3 antibody 67890-1-IgDownload protocol
FC protocol for HSPB3 antibody 67890-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols