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验证数据展示

  • WB analysis using 67935-1-Ig

    WB analysis using 67935-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IF Staining of HeLa using 67935-1-Ig

    IF Staining of HeLa using 67935-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ValRS antibody (67935-1-Ig, Clone: 1A2C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human tonsillitis using 67935-1-Ig

    IHC staining of human tonsillitis using 67935-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67935-1-Ig (ValRS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ValRS Monoclonal antibody

ValRS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67935-1-Ig

产品说明书

CloneNo. 1A2C3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

G7A, Protein G7a, Valine tRNA ligase, ValRS, valyl tRNA synthetase, VARS, VARS1, VARS2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, MCF-7 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67935-1-Ig targets ValRS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 ValRS fusion protein Ag9312 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 valyl-tRNA synthetase
别名 G7A, Protein G7a, Valine tRNA ligase, ValRS, valyl tRNA synthetase, VARS, VARS1, VARS2
计算分子量 1264 aa, 140 kDa
观测分子量 140 kDa
GenBank蛋白编号BC012808
基因名称 ValRS
Gene ID (NCBI) 7407
RRIDAB_2918687
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26640
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ValRS antibody 67935-1-IgDownload protocol
IHC protocol for ValRS antibody 67935-1-IgDownload protocol
IF protocol for ValRS antibody 67935-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols