ValRS Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2C3

Cat no : 67935-1-PBS

Synonyms

G7A, Protein G7a, Valine tRNA ligase, ValRS, valyl tRNA synthetase, VARS, VARS1, VARS2

WB analysis using 67935-1-Ig (same clone as 67935-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67935-1-Ig (same clone as 67935-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ValRS antibody (67935-1-Ig, Clone: 1A2C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67935-1-Ig (same clone as 67935-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67935-1-Ig (ValRS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.

Size

67935-1-PBS targets ValRS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity	Human, mouse, rat
Immunogen	ValRS fusion protein Ag9312 种属同源性预测
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Full Name	valyl-tRNA synthetase
Synonyms	G7A, Protein G7a, Valine tRNA ligase, ValRS, valyl tRNA synthetase, VARS, VARS1, VARS2
Calculated Molecular Weight	1264 aa, 140 kDa
Observed Molecular Weight	140 kDa
GenBank Accession Number	BC012808
Gene Symbol	ValRS
Gene ID (NCBI)	7407
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P26640
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C