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ABCE1 Monoclonal antibody

ABCE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B7E8

Cat no : 67960-1-Ig

Print datasheet

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

验证数据展示

  • WB analysis using 67960-1-Ig

    WB analysis using 67960-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 67960-1-Ig

    WB analysis using 67960-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67960-1-Ig

    IF Staining of U2OS using 67960-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human breast cancer using 67960-1-Ig

    IHC staining of human breast cancer using 67960-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCE1 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, U2OS cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy ABCE1 Ready-To-Use IHC Kit. ABCE1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67960-1-Ig targets ABCE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ABCE1 fusion protein Ag28493 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Synonyms ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I
Calculated Molecular Weight 599 aa, 67 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC016283
Gene Symbol ABCE1
Gene ID (NCBI) 6059
RRIDAB_2918711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.

实验方案

Product Specific Protocols
WB protocol for ABCE1 antibody 67960-1-IgDownload protocol
IHC protocol for ABCE1 antibody 67960-1-IgDownload protocol
IF protocol for ABCE1 antibody 67960-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols