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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

    IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ABCE1 antibody included

ABCE1 Monoclonal antibody, PBS Only

ABCE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67960-1-PBS

产品说明书

CloneNo. 1B7E8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ABCE1 Ready-To-Use IHC Kit. ABCE1 primary antibody included.

产品信息

67960-1-PBS targets ABCE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ABCE1 fusion protein Ag28493 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family E (OABP), member 1
别名 ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I
计算分子量 599 aa, 67 kDa
观测分子量68 kDa
GenBank蛋白编号BC016283
基因名称 ABCE1
Gene ID (NCBI) 6059
RRIDAB_2918711
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61221
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.