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ABCE1 Monoclonal antibody, PBS Only

ABCE1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1B7E8

Cat No : 67960-1-PBS

Print datasheet

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

验证数据展示

  • WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

    IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ABCE1 antibody included
Planning an IHC experiment? We recommend our IHCeasy ABCE1 Ready-To-Use IHC Kit. ABCE1 primary antibody included.

产品信息

67960-1-PBS targets ABCE1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ABCE1 fusion protein Ag28493 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Synonyms ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I
Calculated Molecular Weight 599 aa, 67 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC016283
Gene Symbol ABCE1
Gene ID (NCBI) 6059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61221
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.