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ABCE1 Monoclonal antibody, PBS Only

ABCE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7E8

Cat no : 67960-1-PBS

Print datasheet

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

验证数据展示

  • WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

    IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ABCE1 antibody included
Planning an IHC experiment? We recommend our IHCeasy ABCE1 Ready-To-Use IHC Kit. ABCE1 primary antibody included.

产品信息

67960-1-PBS targets ABCE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ABCE1 fusion protein Ag28493 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Synonyms ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I
Calculated Molecular Weight 599 aa, 67 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC016283
Gene Symbol ABCE1
Gene ID (NCBI) 6059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61221
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.