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验证数据展示

  • WB analysis using 67975-1-Ig

    WB analysis using 67975-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67975-1-Ig

    IF Staining of HeLa using 67975-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARHGEF16 antibody (67975-1-Ig, Clone: 1D8E3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

ARHGEF16 Monoclonal antibody

ARHGEF16 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67975-1-Ig

产品说明书

CloneNo. 1D8E3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

NBR, GEF16, Ephexin-4, EPHEXIN4, Ephexin 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HepG2 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67975-1-Ig targets ARHGEF16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ARHGEF16 fusion protein Ag28675 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Rho guanine exchange factor (GEF) 16
别名 NBR, GEF16, Ephexin-4, EPHEXIN4, Ephexin 4
计算分子量 80 kDa
观测分子量80 kDa
GenBank蛋白编号BC002681
基因名称 ARHGEF16
Gene ID (NCBI) 27237
RRIDAB_2918724
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5VV41
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

实验方案

Product Specific Protocols
WB protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
IF protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols