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ARHGEF16 Monoclonal antibody, PBS Only

ARHGEF16 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat no : 67975-1-PBS

Print datasheet

Synonyms

ARHGEF16, GEF16, NBR

验证数据展示

  • WB analysis using 67975-1-Ig (same clone as 67975-1-PBS)

    WB analysis using 67975-1-Ig (same clone as 67975-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67975-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67975-1-Ig (same clone as 67975-1-PBS)

    IF Staining of MCF-7 using 67975-1-Ig (same clone as 67975-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ARHGEF16 antibody (67975-1-Ig, Clone: 1D8E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67975-1-PBS in a different storage buffer formulation.

产品信息

67975-1-PBS targets ARHGEF16 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ARHGEF16 fusion protein Ag28675 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Rho guanine exchange factor (GEF) 16
Synonyms ARHGEF16, GEF16, NBR
Calculated Molecular Weight 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC002681
Gene Symbol ARHGEF16
Gene ID (NCBI) 27237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5VV41
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.