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ARHGEF16 Monoclonal antibody

ARHGEF16 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat no : 67975-1-Ig

Print datasheet

Synonyms

ARHGEF16, GEF16, NBR

验证数据展示

  • WB analysis using 67975-1-Ig

    WB analysis using 67975-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 67975-1-Ig

    IF Staining of MCF-7 using 67975-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ARHGEF16 antibody (67975-1-Ig, Clone: 1D8E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HepG2 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67975-1-Ig targets ARHGEF16 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen ARHGEF16 fusion protein Ag28675 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Rho guanine exchange factor (GEF) 16
Synonyms ARHGEF16, GEF16, NBR
Calculated Molecular Weight 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC002681
Gene Symbol ARHGEF16
Gene ID (NCBI) 27237
RRIDAB_2918724
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5VV41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

实验方案

Product Specific Protocols
WB protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
IF protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols