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验证数据展示

  • FC experiment of HeLa using CL555-67975

    FC experiment of HeLa using CL555-67975

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human ARHGEF16 (CL555-67975, Clone:1D8E3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®555-conjugated ARHGEF16 Monoclonal antibody

ARHGEF16 Monoclonal Antibody for FC (Intra)
Cat No. CL555-67975

产品说明书

CloneNo. 1D8E3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

FC (Intra)

别名

NBR, GEF16, Ephexin-4, EPHEXIN4, Ephexin 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67975-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67975 targets ARHGEF16 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 ARHGEF16 fusion protein Ag28675 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Rho guanine exchange factor (GEF) 16
别名 NBR, GEF16, Ephexin-4, EPHEXIN4, Ephexin 4
计算分子量 80 kDa
观测分子量80 kDa
GenBank蛋白编号BC002681
基因名称 ARHGEF16
Gene ID (NCBI) 27237
RRIDAB_3084634
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5VV41
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

实验方案

Product Specific Protocols
FC protocol for CL555 ARHGEF16 antibody CL555-67975Download protocol
Standard Protocols
Click here to view our Standard Protocols