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RPA2 Monoclonal antibody

RPA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B9H11

Cat no : 67999-1-Ig

Print datasheet

Synonyms

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34

验证数据展示

  • WB analysis using 67999-1-Ig

    WB analysis using 67999-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of A431 using 67999-1-Ig

    IF Staining of A431 using 67999-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

经过测试的应用

Positive WB detected inLNCaP cells, pig spleen tissue, rat testis tissue, mouse testis tissue, rabbit spleen tissue, Hela cells, MOLT-4 cells, Jurkat cells, THP-1 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67999-1-Ig targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig, rabbit
Immunogen RPA2 fusion protein Ag24783 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication protein A2, 32kDa
Synonyms REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34
Calculated Molecular Weight 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
RRIDAB_2918747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15927
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.

实验方案

Product Specific Protocols
WB protocol for RPA2 antibody 67999-1-IgDownload protocol
IF protocol for RPA2 antibody 67999-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols