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RPA2 Monoclonal antibody, PBS Only

RPA2 Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, rabbit

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1B9H11

Cat No : 67999-1-PBS

Print datasheet

Synonyms

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34

验证数据展示

  • WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

产品信息

67999-1-PBS targets RPA2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig, rabbit
Immunogen RPA2 fusion protein Ag24783 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication protein A2, 32kDa
Synonyms REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34
Calculated Molecular Weight 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15927
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.