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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

RPA2 Monoclonal antibody, PBS Only

RPA2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67999-1-PBS
CloneNo. 1B9H11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

67999-1-PBS targets RPA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen RPA2 fusion protein Ag24783 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication protein A2, 32kDa
Synonyms RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
RRIDAB_2918747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15927
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.