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RPA2 Monoclonal antibody, PBS Only

RPA2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9H11

Cat no : 67999-1-PBS

Print datasheet

Synonyms

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34

验证数据展示

  • WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

产品信息

67999-1-PBS targets RPA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig, rabbit
Immunogen RPA2 fusion protein Ag24783 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication protein A2, 32kDa
Synonyms REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34
Calculated Molecular Weight 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15927
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.