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验证数据展示

  • WB analysis using 68130-1-Ig

    WB analysis using 68130-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of RAW 264.7 using 68130-1-Ig

    WB analysis of RAW 264.7 using 68130-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

RYBP Monoclonal antibody

RYBP Monoclonal Antibody for WB, ELISA
Cat No. 68130-1-Ig

产品说明书

CloneNo. 2C6G7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, ELISA

别名

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, RAW 264.7 cells, HeLa cells, U2OS cells, HEK-293 cells, LNCaP cells, Jurkat cells, Daudi cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68130-1-Ig targets RYBP in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 RYBP fusion protein Ag31715 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RING1 and YY1 binding protein
别名 AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1
计算分子量 228 aa, 25 kDa
观测分子量32 kDa
GenBank蛋白编号BC014959
基因名称 RYBP
Gene ID (NCBI) 23429
RRIDAB_2923657
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N488
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

实验方案

Product Specific Protocols
WB protocol for RYBP antibody 68130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols