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验证数据展示

  • FC experiment of A431 using CL488-68130

    FC experiment of A431 using CL488-68130

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RYBP (CL488-68130, Clone:2C6G7) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated RYBP Monoclonal antibody

RYBP Monoclonal Antibody for FC (Intra)
Cat No. CL488-68130

产品说明书

CloneNo. 2C6G7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

FC (Intra)

别名

RYBP, YEAF1, YY1 and E4TF1-associated factor 1, 2C6G7, AAP1, APAP 1, APAP-1, Apoptin-associating protein 1, Death effector domain-associated factor, DED associated factor, DEDAF, DED-associated factor, RING1 and YY1-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68130-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68130 targets RYBP in FC (Intra) applications and shows reactivity with Human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse
免疫原 RYBP fusion protein Ag31715 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RING1 and YY1 binding protein
别名 RYBP, YEAF1, YY1 and E4TF1-associated factor 1, 2C6G7, AAP1, APAP 1, APAP-1, Apoptin-associating protein 1, Death effector domain-associated factor, DED associated factor, DEDAF, DED-associated factor, RING1 and YY1-binding protein
计算分子量 228 aa, 25 kDa
观测分子量32 kDa
GenBank蛋白编号BC014959
基因名称 RYBP
Gene ID (NCBI) 23429
RRIDAB_3084411
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N488
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RYBP antibody CL488-68130Download protocol
Standard Protocols
Click here to view our Standard Protocols