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RYBP Monoclonal antibody

RYBP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C6G7

Cat no : 68130-1-Ig

Print datasheet

Synonyms

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

验证数据展示

  • WB analysis using 68130-1-Ig

    WB analysis using 68130-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of RAW 264.7 using 68130-1-Ig

    WB analysis of RAW 264.7 using 68130-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, RAW 264.7 cells, HeLa cells, U2OS cells, HEK-293 cells, LNCaP cells, Jurkat cells, Daudi cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68130-1-Ig targets RYBP in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen RYBP fusion protein Ag31715 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RING1 and YY1 binding protein
Synonyms AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC014959
Gene Symbol RYBP
Gene ID (NCBI) 23429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

实验方案

Product Specific Protocols
WB protocol for RYBP antibody 68130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols