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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68130-1-Ig (same clone as 68130-1-PBS)

    WB analysis using 68130-1-Ig (same clone as 68130-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68130-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 68130-1-Ig (same clone as 68130-1-PBS)

    WB analysis of RAW 264.7 using 68130-1-Ig (same clone as 68130-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68130-1-PBS in a different storage buffer formulation.

RYBP Monoclonal antibody, PBS Only

RYBP Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68130-1-PBS

产品说明书

CloneNo. 2C6G7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, Indirect ELISA

别名

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68130-1-PBS targets RYBP in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 RYBP fusion protein Ag31715 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RING1 and YY1 binding protein
别名 AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1
计算分子量 228 aa, 25 kDa
观测分子量32 kDa
GenBank蛋白编号BC014959
基因名称 RYBP
Gene ID (NCBI) 23429
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N488
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]