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DFFA,DFF45 Monoclonal antibody, PBS Only (Capture/Detector)

DFFA,DFF45 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F6H9

Cat no : 68177-1-PBS

Print datasheet

Synonyms

DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1

验证数据展示

  • WB analysis using 68177-1-Ig (same clone as 68177-1-PBS)

    WB analysis using 68177-1-Ig (same clone as 68177-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68177-1-Ig (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    IF Staining of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DFFA,DFF45 antibody (68177-1-Ig, Clone: 1F6H9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    FC experiment of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug DFFA,DFF45 Monoclonal antibody (68177-1-Ig, Clone:1F6H9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50354-1

    Cytometric bead array standard curve of MP50354-1

    Cytometric bead array standard curve of MP50354-1, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-1-PBS. Detection antibody: 68177-2-PBS. Standard:Ag32657. Range: 3.125-200 ng/mL.

  • Cytometric bead array standard curve of MP50354-2

    Cytometric bead array standard curve of MP50354-2

    Cytometric bead array standard curve of MP50354-2, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-3-PBS. Detection antibody: 68177-1-PBS. Standard:Ag32657. Range: 6.25-200 ng/mL.

产品信息

68177-1-PBS targets DFFA,DFF45 as part of a matched antibody pair:

MP50354-1: 68177-1-PBS capture and 68177-2-PBS detection (validated in Cytometric bead array)

MP50354-2: 68177-3-PBS capture and 68177-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DFFA,DFF45 fusion protein Ag32657 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DNA fragmentation factor, 45kDa, alpha polypeptide
Synonyms DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC000037
Gene Symbol DFFA
Gene ID (NCBI) 1676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00273
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.