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ENOPH1 Monoclonal antibody, PBS Only (Capture)

ENOPH1 Monoclonal Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

1F1E11

Cat no : 68179-1-PBS

Print datasheet

Synonyms

MASA, Enolase-phosphatase E1, EC:3.1.3.77, E1, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

验证数据展示

  • WB analysis using 68179-1-Ig (same clone as 68179-1-PBS)

    WB analysis using 68179-1-Ig (same clone as 68179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68179-1-Ig (ENOPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68179-1-Ig (same clone as 68179-1-PBS)

    FC experiment of HepG2 using 68179-1-Ig (same clone as 68179-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ENOPH1 Monoclonal antibody (68179-1-Ig, Clone:1F1E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50127-1

    Cytometric bead array standard curve of MP50127-1

    Cytometric bead array standard curve of MP50127-1, ENOPH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68179-1-PBS. Detection antibody: 68179-2-PBS. Standard:Ag12553. Range: 0.781-100 ng/mL.

产品信息

68179-1-PBS targets ENOPH1 as part of a matched antibody pair:

MP50127-1: 68179-1-PBS capture and 68179-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen ENOPH1 fusion protein Ag12553 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name enolase-phosphatase 1
Synonyms MASA, Enolase-phosphatase E1, EC:3.1.3.77, E1, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight29-35 kDa
GenBank Accession NumberBC065815
Gene Symbol ENOPH1
Gene ID (NCBI) 58478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHY7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.