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验证数据展示

  • WB analysis using 68179-1-Ig (same clone as 68179-1-PBS)

    WB analysis using 68179-1-Ig (same clone as 68179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68179-1-Ig (ENOPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68179-1-Ig (same clone as 68179-1-PBS)

    FC experiment of HepG2 using 68179-1-Ig (same clone as 68179-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ENOPH1 Monoclonal antibody (68179-1-Ig, Clone:1F1E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50127-1

    Cytometric bead array standard curve of MP50127-1

    Cytometric bead array standard curve of MP50127-1, ENOPH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68179-1-PBS. Detection antibody: 68179-2-PBS. Standard:Ag12553. Range: 0.781-100 ng/mL.

ENOPH1 Monoclonal antibody, PBS Only (Capture)

ENOPH1 Monoclonal Antibody for WB, Indirect ELISA, Cytometric bead array
Cat No. 68179-1-PBS

产品说明书

CloneNo. 1F1E11

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit, Pig

应用

WB, Indirect ELISA, Cytometric bead array

别名

MASA, Enolase-phosphatase E1, EC:3.1.3.77, E1, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68179-1-PBS targets ENOPH1 as part of a matched antibody pair:

MP50127-1: 68179-1-PBS capture and 68179-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Indirect ELISA, Cytometric bead array Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig
免疫原 ENOPH1 fusion protein Ag12553 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 enolase-phosphatase 1
别名 MASA, Enolase-phosphatase E1, EC:3.1.3.77, E1, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
计算分子量 261 aa, 29 kDa
观测分子量29-35 kDa
GenBank蛋白编号BC065815
基因名称 ENOPH1
Gene ID (NCBI) 58478
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UHY7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.