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验证数据展示

  • WB analysis using 68208-1-Ig (same clone as 68208-1-PBS)

    WB analysis using 68208-1-Ig (same clone as 68208-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

  • WB analysis of rat heart using 68208-1-Ig (same clone as 68208-1-PBS)

    WB analysis of rat heart using 68208-1-Ig (same clone as 68208-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 68208-1-Ig (same clone as 68208-1-PBS)

    IF Staining of U-251 using 68208-1-Ig (same clone as 68208-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68208-1-Ig (same clone as 68208-1-PBS)

    IF Staining of HeLa using 68208-1-Ig (same clone as 68208-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

  • IHC staining of rat adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    IHC staining of rat adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LETM1 antibody included
  • IHC staining of rat adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    IHC staining of rat adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LETM1 antibody included
  • IF Staining of HeLa using 68208-1-Ig (same clone as 68208-1-PBS)

    IF Staining of HeLa using 68208-1-Ig (same clone as 68208-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    IHC staining of mouse adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LETM1 antibody included
  • IHC staining of mouse adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    IHC staining of mouse adrenal gland using 68208-1-Ig (same clone as 68208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LETM1 antibody included
  • Cytometric bead array standard curve of MP50653-2

    Cytometric bead array standard curve of MP50653-2

    Cytometric bead array standard curve of MP50653-2, LETM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68208-4-PBS. Detection antibody: 68208-1-PBS. Standard:Ag8851. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO验证

LETM1 Monoclonal antibody, PBS Only (Detector)

LETM1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68208-1-PBS

产品说明书

CloneNo. 2B9B9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Leucine zipper-EF-hand-containing transmembrane protein 1, 2B9B9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LETM1 Ready-To-Use IHC Kit. LETM1 primary antibody included.

产品信息

68208-1-PBS targets LETM1 as part of a matched antibody pair:

MP50653-2: 68208-4-PBS capture and 68208-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 LETM1 fusion protein Ag8851 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 leucine zipper-EF-hand containing transmembrane protein 1
别名 Leucine zipper-EF-hand-containing transmembrane protein 1, 2B9B9
计算分子量 739 aa, 83 kDa
观测分子量83 kDa
GenBank蛋白编号BC021208
基因名称 LETM1
Gene ID (NCBI) 3954
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO95202
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.