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NF90/ILF3 Monoclonal antibody, PBS Only (Capture)

NF90/ILF3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H10G3

Cat no : 68213-1-PBS

Print datasheet

Synonyms

ILF3, IL-F3, DRBP76, DRBF, CBTF

验证数据展示

  • WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

    WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

  • WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

    WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 68213-1-Ig (same clone as 68213-1-PBS)

    IHC staining of mouse eye using 68213-1-Ig (same clone as 68213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ILF3 antibody included
  • Cytometric bead array standard curve of MP50472-3

    Cytometric bead array standard curve of MP50472-3

    Cytometric bead array standard curve of MP50472-3, NF90,ILF3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68213-1-PBS. Detection antibody: 68213-4-PBS. Standard:Ag30868. Range: 0.781-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy ILF3 Ready-To-Use IHC Kit. ILF3 primary antibody included.

产品信息

68213-1-PBS targets NF90/ILF3 as part of a matched antibody pair:

MP50472-3: 68213-1-PBS capture and 68213-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NF90/ILF3 fusion protein Ag30868 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin enhancer binding factor 3, 90kDa
Synonyms ILF3, IL-F3, DRBP76, DRBF, CBTF
Calculated Molecular Weight 894 aa, 95 kDa
Observed Molecular Weight90 kDa, 110 kDa
GenBank Accession NumberBC064836
Gene Symbol ILF3
Gene ID (NCBI) 3609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12906
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C