验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, pig cerebellum tissue, pig brain tissue, rabbit brain tissue, rabbit cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68252-1-Ig targets CNTN1 in WB, ELISA applications and shows reactivity with Human, pig, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, pig, rabbit |
| 免疫原 | CNTN1 fusion protein Ag5030 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | contactin 1 |
| 别名 | CNTN1, contactin 1, F3, Glycoprotein gp135, GP135, Neural cell surface protein F3 |
| 计算分子量 | 1018 aa, 113 kDa |
| 观测分子量 | 130-135 kDa |
| GenBank蛋白编号 | BC036569 |
| 基因名称 | CNTN1 |
| Gene ID (NCBI) | 1272 |
| RRID | AB_2935337 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q12860 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNTN1 antibody 68252-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
