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RPS20 Monoclonal antibody

RPS20 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E9B4

Cat no : 68270-1-Ig

Print datasheet

Synonyms

40S ribosomal protein S20, FLJ27451, ribosomal protein S20, RPS20

验证数据展示

  • WB analysis using 68270-1-Ig

    WB analysis using 68270-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68270-1-Ig

    WB analysis using 68270-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 68270-1-Ig

    IHC staining of human breast cancer using 68270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with RPS20 antibody included
  • IF Staining of HeLa using 68270-1-Ig

    IF Staining of HeLa using 68270-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS20 Ready-To-Use IHC Kit. RPS20 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68270-1-Ig targets RPS20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RPS20 fusion protein Ag8275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein S20
Synonyms 40S ribosomal protein S20, FLJ27451, ribosomal protein S20, RPS20
Calculated Molecular Weight 13 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC007507
Gene Symbol RPS20
Gene ID (NCBI) 6224
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60866
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

实验方案

Product Specific Protocols
WB protocol for RPS20 antibody 68270-1-IgDownload protocol
IHC protocol for RPS20 antibody 68270-1-IgDownload protocol
IF protocol for RPS20 antibody 68270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols