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RPS20 Monoclonal antibody, PBS Only (Detector)

RPS20 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E9B4

Cat no : 68270-1-PBS

Print datasheet

Synonyms

Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4

验证数据展示

  • WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)

    IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS20 antibody included
  • IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)

    IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50774-1

    Cytometric bead array standard curve of MP50774-1

    Cytometric bead array standard curve of MP50774-1, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-2-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 1.563-200 ng/mL.

  • Cytometric bead array standard curve of MP50774-2

    Cytometric bead array standard curve of MP50774-2

    Cytometric bead array standard curve of MP50774-2, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-3-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 3.125-200 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy RPS20 Ready-To-Use IHC Kit. RPS20 primary antibody included.

产品信息

68270-1-PBS targets RPS20 as part of a matched antibody pair:

MP50774-1: 68270-2-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

MP50774-2: 68270-3-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPS20 fusion protein Ag8275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein S20
Synonyms Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4
Calculated Molecular Weight 13 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC007507
Gene Symbol RPS20
Gene ID (NCBI) 6224
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60866
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.