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验证数据展示

  • WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)

    IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS20 antibody included
  • IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)

    IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50774-1

    Cytometric bead array standard curve of MP50774-1

    Cytometric bead array standard curve of MP50774-1, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-2-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 1.563-200 ng/mL.

  • Cytometric bead array standard curve of MP50774-2

    Cytometric bead array standard curve of MP50774-2

    Cytometric bead array standard curve of MP50774-2, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-3-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 3.125-200 ng/mL.

RPS20 Monoclonal antibody, PBS Only (Detector)

RPS20 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68270-1-PBS

产品说明书

CloneNo. 3E9B4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RPS20 Ready-To-Use IHC Kit. RPS20 primary antibody included.

产品信息

68270-1-PBS targets RPS20 as part of a matched antibody pair:

MP50774-1: 68270-2-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

MP50774-2: 68270-3-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPS20 fusion protein Ag8275 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S20
别名 Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4
计算分子量 13 kDa
观测分子量16 kDa
GenBank蛋白编号BC007507
基因名称 RPS20
Gene ID (NCBI) 6224
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60866
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.