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验证数据展示

  • WB analysis using 68274-1-Ig

    WB analysis using 68274-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of NIH/3T3 using 68274-1-Ig

    WB analysis of NIH/3T3 using 68274-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 68274-1-Ig

    FC experiment of MCF-7 using 68274-1-Ig

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug DHFR Monoclonal antibody (68274-1-Ig, Clone:4C11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DHFR Monoclonal antibody

DHFR Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68274-1-Ig

产品说明书

CloneNo. 4C11E9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

EC:1.5.1.3, DHFRP1, 4C11E9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68274-1-Ig targets DHFR in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 DHFR fusion protein Ag7318 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydrofolate reductase
别名 EC:1.5.1.3, DHFRP1, 4C11E9
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC000192
基因名称 DHFR
Gene ID (NCBI) 1719
RRIDAB_2935357
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00374
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

实验方案

Product Specific Protocols
WB protocol for DHFR antibody 68274-1-IgDownload protocol
FC protocol for DHFR antibody 68274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols