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DHFR Monoclonal antibody, PBS Only

DHFR Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

4C11E9

Cat no : 68274-1-PBS

Print datasheet

Synonyms

EC:1.5.1.3, DHFRP1, 4C11E9

验证数据展示

  • WB analysis using 68274-1-Ig (same clone as 68274-1-PBS)

    WB analysis using 68274-1-Ig (same clone as 68274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68274-1-Ig (same clone as 68274-1-PBS)

    WB analysis of NIH/3T3 using 68274-1-Ig (same clone as 68274-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 68274-1-Ig (same clone as 68274-1-PBS)

    FC experiment of MCF-7 using 68274-1-Ig (same clone as 68274-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug DHFR Monoclonal antibody (68274-1-Ig, Clone:4C11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

产品信息

68274-1-PBS targets DHFR in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity mouse, human
Immunogen DHFR fusion protein Ag7318 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dihydrofolate reductase
Synonyms EC:1.5.1.3, DHFRP1, 4C11E9
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00374
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).