• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68274-1-Ig (same clone as 68274-1-PBS)

    WB analysis using 68274-1-Ig (same clone as 68274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68274-1-Ig (same clone as 68274-1-PBS)

    WB analysis of NIH/3T3 using 68274-1-Ig (same clone as 68274-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 68274-1-Ig (same clone as 68274-1-PBS)

    FC experiment of MCF-7 using 68274-1-Ig (same clone as 68274-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug DHFR Monoclonal antibody (68274-1-Ig, Clone:4C11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

DHFR Monoclonal antibody, PBS Only

DHFR Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68274-1-PBS

产品说明书

CloneNo. 4C11E9

宿主/亚型

Mouse / IgG1

种属反应性

mouse, human

应用

Indirect ELISA, WB

别名

EC:1.5.1.3, DHFRP1, 4C11E9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

68274-1-PBS targets DHFR in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 mouse, human
免疫原 DHFR fusion protein Ag7318 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydrofolate reductase
别名 EC:1.5.1.3, DHFRP1, 4C11E9
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC000192
基因名称 DHFR
Gene ID (NCBI) 1719
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00374
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).