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验证数据展示

  • WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68321-1-Ig (same clone as 68321-1-PBS)

    FC experiment of HepG2 using 68321-1-Ig (same clone as 68321-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MTHFD1L Monoclonal antibody (68321-1-Ig, Clone:1G1F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50271-2

    Cytometric bead array standard curve of MP50271-2

    Cytometric bead array standard curve of MP50271-2, MTHFD1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68321-1-PBS. Detection antibody: 68321-3-PBS. Standard:Ag9061. Range: 0.195-50 ng/mL.

MTHFD1L Monoclonal antibody, PBS Only (Capture)

MTHFD1L Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 68321-1-PBS

产品说明书

CloneNo. 1G1F2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3, 1G1F2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68321-1-PBS targets MTHFD1L as part of a matched antibody pair:

MP50271-2: 68321-1-PBS capture and 68321-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 MTHFD1L fusion protein Ag9061 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
别名 MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3, 1G1F2
计算分子量 978 aa, 106 kDa
观测分子量106 kDa
GenBank蛋白编号BC017477
基因名称 MTHFD1L
Gene ID (NCBI) 25902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6UB35
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.