• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ALAS2 Monoclonal antibody, PBS Only (Capture)

ALAS2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1H1

Cat no : 68332-1-PBS

Print datasheet

Synonyms

ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2, 5-aminolevulinate synthase, erythroid-specific, mitochondrial

验证数据展示

  • WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • WB analysis of NK-92 using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis of NK-92 using 68332-1-Ig (same clone as 68332-1-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50624-1

    Cytometric bead array standard curve of MP50624-1

    Cytometric bead array standard curve of MP50624-1, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-2-PBS. Standard:Ag13524. Range: 6.25-100 ng/mL.

  • Cytometric bead array standard curve of MP50624-2

    Cytometric bead array standard curve of MP50624-2

    Cytometric bead array standard curve of MP50624-2, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-3-PBS. Standard:Ag13524. Range: 3.125-100 ng/mL.

产品信息

68332-1-PBS targets ALAS2 as part of a matched antibody pair:

MP50624-1: 68332-1-PBS capture and 68332-2-PBS detection (validated in Cytometric bead array)

MP50624-2: 68332-1-PBS capture and 68332-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ALAS2 fusion protein Ag13524 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aminolevulinate, delta-, synthase 2
Synonyms ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2, 5-aminolevulinate synthase, erythroid-specific, mitochondrial
Calculated Molecular Weight 587 aa, 65 kDa
Observed Molecular Weight60~65 kDa
GenBank Accession NumberBC030230
Gene Symbol ALAS2
Gene ID (NCBI) 212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22557
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.