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验证数据展示

  • FC experiment of Jurkat using 68395-1-Ig

    FC experiment of Jurkat using 68395-1-Ig

    1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed.

  • IF Staining of NCCIT using 68395-1-Ig

    IF Staining of NCCIT using 68395-1-Ig

    Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for IF/ICC, FC, ELISA
Cat No. 68395-1-Ig

产品说明书

CloneNo. 1H3F4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC, ELISA

别名

B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNCCIT cells, A431 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC)FC : 1.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68395-1-Ig targets Beta-2-Microglobulin in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 beta-2-microglobulin
别名 B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3
计算分子量 119 aa, 14 kDa
观测分子量 12-14 kDa
GenBank蛋白编号BC032589
基因名称 B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_3085112
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61769
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.

实验方案

Product Specific Protocols
IF protocol for Beta-2-Microglobulin antibody 68395-1-IgDownload protocol
FC protocol for Beta-2-Microglobulin antibody 68395-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols