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Beta-2-Microglobulin Monoclonal antibody, PBS Only

Beta-2-Microglobulin Monoclonal Antibody for IF/ICC, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H3F4

Cat no : 68395-1-PBS

Print datasheet

Synonyms

B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

验证数据展示

  • FC experiment of Jurkat using 68395-1-Ig (same clone as 68395-1-PBS)

    FC experiment of Jurkat using 68395-1-Ig (same clone as 68395-1-PBS)

    1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed. This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

  • IF Staining of NCCIT using 68395-1-Ig (same clone as 68395-1-PBS)

    IF Staining of NCCIT using 68395-1-Ig (same clone as 68395-1-PBS)

    Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

产品信息

68395-1-PBS targets Beta-2-Microglobulin in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name beta-2-microglobulin
Synonyms B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3
Calculated Molecular Weight 119 aa, 14 kDa
Observed Molecular Weight 12-14 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61769
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.