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验证数据展示

  • FC experiment of Jurkat using 68395-1-Ig

    FC experiment of Jurkat using 68395-1-Ig

    1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed.

  • IF Staining of NCCIT using 68395-1-Ig

    IF Staining of NCCIT using 68395-1-Ig

    Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for IF/ICC, FC, ELISA
Cat No. 68395-1-Ig

Print datasheet

CloneNo. 1H3F4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, ELISA

Synonyms

B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNCCIT cells, A431 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC)FC : 1.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68395-1-Ig targets Beta-2-Microglobulin in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name beta-2-microglobulin
Synonyms B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3
Calculated Molecular Weight 119 aa, 14 kDa
Observed Molecular Weight 12-14 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_3085112
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.

实验方案

Product Specific Protocols
IF protocol for Beta-2-Microglobulin antibody 68395-1-IgDownload protocol
FC protocol for Beta-2-Microglobulin antibody 68395-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols