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GATC Monoclonal antibody

GATC Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F9C8

Cat no : 68405-1-Ig

Print datasheet

Synonyms

15E1.2, FLJ37000, GATC, GatC like protein, Protein 15E1.2

验证数据展示

  • WB analysis of A549 using 68405-1-Ig

    WB analysis of A549 using 68405-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68405-1-Ig

    WB analysis using 68405-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, HEK-293 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68405-1-Ig targets GATC in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen GATC fusion protein Ag19857 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
Synonyms 15E1.2, FLJ37000, GATC, GatC like protein, Protein 15E1.2
Calculated Molecular Weight 136 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC107145
Gene Symbol GATC
Gene ID (NCBI) 283459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43716
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).

实验方案

Product Specific Protocols
WB protocol for GATC antibody 68405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols