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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of HEK-293 using 68405-1-Ig (same clone as 68405-1-PBS)

    FC experiment of HEK-293 using 68405-1-Ig (same clone as 68405-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATC Monoclonal antibody (68405-1-Ig, Clone:2F9C8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68405-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 68405-1-Ig (same clone as 68405-1-PBS)

    WB analysis of A549 using 68405-1-Ig (same clone as 68405-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68405-1-PBS in a different storage buffer formulation.

  • WB analysis using 68405-1-Ig (same clone as 68405-1-PBS)

    WB analysis using 68405-1-Ig (same clone as 68405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68405-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68405-1-Ig (same clone as 68405-1-PBS)

    IF Staining of HEK-293 using 68405-1-Ig (same clone as 68405-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GATC antibody (68405-1-Ig, Clone: 2F9C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68405-1-PBS in a different storage buffer formulation.

GATC Monoclonal antibody, PBS Only

GATC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68405-1-PBS

产品说明书

CloneNo. 2F9C8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Protein 15E1.2, Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial, Glu-AdT subunit C, GatC like protein, EC:6.3.5.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68405-1-PBS targets GATC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 GATC fusion protein Ag19857 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
别名 Protein 15E1.2, Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial, Glu-AdT subunit C, GatC like protein, EC:6.3.5.-
计算分子量 136 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC107145
基因名称 GATC
Gene ID (NCBI) 283459
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43716
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).