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验证数据展示

  • WB analysis using 68423-1-Ig

    WB analysis using 68423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68423-1-Ig

    IF Staining of HeLa using 68423-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SSSCA1 Monoclonal antibody

SSSCA1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68423-1-Ig

产品说明书

CloneNo. 1D2E8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Autoantigen p27, p27, SSSCA1, ZNRD2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68423-1-Ig targets SSSCA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 SSSCA1 fusion protein Ag16783 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Sjogren syndrome/scleroderma autoantigen 1
别名 Autoantigen p27, p27, SSSCA1, ZNRD2
计算分子量 199 aa, 21 kDa
观测分子量23 kDa
GenBank蛋白编号BC014791
基因名称 SSSCA1
Gene ID (NCBI) 10534
RRIDAB_3085138
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60232
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

实验方案

Product Specific Protocols
WB protocol for SSSCA1 antibody 68423-1-IgDownload protocol
IF protocol for SSSCA1 antibody 68423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols