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SSSCA1 Monoclonal antibody, PBS Only

SSSCA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2E8

Cat no : 68423-1-PBS

Print datasheet

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

验证数据展示

  • WB analysis using 68423-1-Ig (same clone as 68423-1-PBS)

    WB analysis using 68423-1-Ig (same clone as 68423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68423-1-Ig (same clone as 68423-1-PBS)

    IF Staining of HeLa using 68423-1-Ig (same clone as 68423-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

产品信息

68423-1-PBS targets SSSCA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SSSCA1 fusion protein Ag16783 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Sjogren syndrome/scleroderma autoantigen 1
Synonyms Autoantigen p27, p27, SSSCA1, ZNRD2
Calculated Molecular Weight 199 aa, 21 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC014791
Gene Symbol SSSCA1
Gene ID (NCBI) 10534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60232
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.