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SSSCA1 Monoclonal antibody

SSSCA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2E8

Cat no : 68423-1-Ig

Print datasheet

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

验证数据展示

  • WB analysis using 68423-1-Ig

    WB analysis using 68423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68423-1-Ig

    IF Staining of HeLa using 68423-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68423-1-Ig targets SSSCA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SSSCA1 fusion protein Ag16783 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Sjogren syndrome/scleroderma autoantigen 1
Synonyms Autoantigen p27, p27, SSSCA1, ZNRD2
Calculated Molecular Weight 199 aa, 21 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC014791
Gene Symbol SSSCA1
Gene ID (NCBI) 10534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60232
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

实验方案

Product Specific Protocols
WB protocol for SSSCA1 antibody 68423-1-IgDownload protocol
IF protocol for SSSCA1 antibody 68423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols