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验证数据展示

  • IF Staining of HeLa using CL488-68423

    IF Staining of HeLa using CL488-68423

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 SSSCA1 antibody (CL488-68423, Clone: 1D2E8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68423

    FC experiment of HeLa using CL488-68423

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SSSCA1 Monoclonal antibody (CL488-68423, Clone:1D2E8)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SSSCA1 Monoclonal antibody

SSSCA1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68423

产品说明书

CloneNo. 1D2E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

ZNRD2, Zinc ribbon domain-containing protein 2, Protein ZNRD2, p27, Autoantigen p27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68423-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68423 targets SSSCA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SSSCA1 fusion protein Ag16783 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Sjogren syndrome/scleroderma autoantigen 1
别名 ZNRD2, Zinc ribbon domain-containing protein 2, Protein ZNRD2, p27, Autoantigen p27
计算分子量 199 aa, 21 kDa
观测分子量23 kDa
GenBank蛋白编号BC014791
基因名称 SSSCA1
Gene ID (NCBI) 10534
RRIDAB_3673012
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60232
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SSSCA1 antibody CL488-68423Download protocol
FC protocol for CL Plus 488 SSSCA1 antibody CL488-68423Download protocol
Standard Protocols
Click here to view our Standard Protocols