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验证数据展示

  • WB analysis using 68443-1-Ig

    WB analysis using 68443-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 68443-1-Ig

    IP experiment of HeLa using 68443-1-Ig

    IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug.

  • IF Staining of HeLa using 68443-1-Ig

    IF Staining of HeLa using 68443-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

VPS4B Monoclonal antibody

VPS4B Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68443-1-Ig

产品说明书

CloneNo. 2H3G7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse , rat

应用

WB, IF/ICC, IP, ELISA

别名

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68443-1-Ig targets VPS4B in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse , rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse , rat
免疫原 VPS4B fusion protein Ag11610 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 4 homolog B (S. cerevisiae)
别名 MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B
计算分子量 444 aa, 49 kDa
观测分子量~50 kDa
GenBank蛋白编号BC039574
基因名称 VPS4B
Gene ID (NCBI) 9525
RRIDAB_3085156
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75351
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)

实验方案

Product Specific Protocols
WB protocol for VPS4B antibody 68443-1-IgDownload protocol
IF protocol for VPS4B antibody 68443-1-IgDownload protocol
IP protocol for VPS4B antibody 68443-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols