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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68443-1-Ig (same clone as 68443-1-PBS)

    WB analysis using 68443-1-Ig (same clone as 68443-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    IP experiment of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    IF Staining of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

VPS4B Monoclonal antibody, PBS Only

VPS4B Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 68443-1-PBS

产品说明书

CloneNo. 2H3G7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse , rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68443-1-PBS targets VPS4B in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, mouse , rat
免疫原 VPS4B fusion protein Ag11610 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 4 homolog B (S. cerevisiae)
别名 MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B
计算分子量 444 aa, 49 kDa
观测分子量~50 kDa
GenBank蛋白编号BC039574
基因名称 VPS4B
Gene ID (NCBI) 9525
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75351
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)