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VPS4B Monoclonal antibody, PBS Only

VPS4B Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3G7

Cat no : 68443-1-PBS

Print datasheet

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

验证数据展示

  • WB analysis using 68443-1-Ig (same clone as 68443-1-PBS)

    WB analysis using 68443-1-Ig (same clone as 68443-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    IP experiment of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    IF Staining of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

产品信息

68443-1-PBS targets VPS4B in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, mouse , rat
Immunogen VPS4B fusion protein Ag11610 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Synonyms MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B
Calculated Molecular Weight 444 aa, 49 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC039574
Gene Symbol VPS4B
Gene ID (NCBI) 9525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75351
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)