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验证数据展示

  • WB analysis using 68443-1-Ig

    WB analysis using 68443-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 68443-1-Ig

    IP experiment of HeLa using 68443-1-Ig

    IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug.

  • IF Staining of HeLa using 68443-1-Ig

    IF Staining of HeLa using 68443-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

VPS4B Monoclonal antibody

VPS4B Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68443-1-Ig

Print datasheet

CloneNo. 2H3G7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68443-1-Ig targets VPS4B in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse , rat
Immunogen VPS4B fusion protein Ag11610 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Synonyms MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B
Calculated Molecular Weight 444 aa, 49 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC039574
Gene Symbol VPS4B
Gene ID (NCBI) 9525
RRIDAB_3085156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75351
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)

实验方案

Product Specific Protocols
WB protocol for VPS4B antibody 68443-1-IgDownload protocol
IF protocol for VPS4B antibody 68443-1-IgDownload protocol
IP protocol for VPS4B antibody 68443-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols