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CoraLite® Plus 488-conjugated VPS4B Monoclonal antibody

VPS4B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H3G7

Cat No : CL488-68443

Print datasheet

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

验证数据展示

  • IF Staining of HeLa using CL488-68443

    IF Staining of HeLa using CL488-68443

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 VPS4B antibody (CL488-68443, Clone: 2H3G7 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68443-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68443 targets VPS4B in IF applications and shows reactivity with Human, mouse , rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse , rat
Immunogen VPS4B fusion protein Ag11610 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Synonyms MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B
Calculated Molecular Weight 444 aa, 49 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC039574
Gene Symbol VPS4B
Gene ID (NCBI) 9525
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75351
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VPS4B antibody CL488-68443Download protocol
Standard Protocols
Click here to view our Standard Protocols