验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 68443-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68443 targets VPS4B in IF/ICC applications and shows reactivity with Human, mouse , rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse , rat |
| 免疫原 | VPS4B fusion protein Ag11610 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 4 homolog B (S. cerevisiae) |
| 别名 | MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B |
| 计算分子量 | 444 aa, 49 kDa |
| 观测分子量 | ~50 kDa |
| GenBank蛋白编号 | BC039574 |
| 基因名称 | VPS4B |
| Gene ID (NCBI) | 9525 |
| RRID | AB_3673016 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O75351 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VPS4B antibody CL488-68443 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |