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N-cadherin Monoclonal antibody, PBS Only (Detector)

N-cadherin Monoclonal Antibody for WB, IF/ICC, FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D9G1

Cat no : 68532-2-PBS

Print datasheet

Synonyms

CDHN, CDH2, CD325, Cadherin-2, Cadherin 2

验证数据展示

  • WB analysis using 68532-2-Ig (same clone as 68532-2-PBS)

    WB analysis using 68532-2-Ig (same clone as 68532-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68532-2-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68532-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68532-2-Ig (same clone as 68532-2-PBS)

    IF Staining of HepG2 using 68532-2-Ig (same clone as 68532-2-PBS)

    Immunofluorescent analysis of un-fixed HepG2 cells using N-cadherin antibody (68532-2-Ig, Clone: 2D9G1 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68532-2-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 68532-2-Ig (same clone as 68532-2-PBS)

    FC experiment of SH-SY5Y using 68532-2-Ig (same clone as 68532-2-PBS)

    1x10^6 MCF-7 (red, dashed) and SH-SY5Y (red, filled) were surface stained with 0.2 μg N-cadherin Monoclonal antibody (68532-2-Ig, Clone: 2D9G1) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were not fixed. This data was developed using the same antibody clone with 68532-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50439-1

    Cytometric bead array standard curve of MP50439-1

    Cytometric bead array standard curve of MP50439-1, N-cadherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66219-2-PBS. Detection antibody: 68532-2-PBS. Standard:Eg0701. Range: 0.781-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

产品信息

68532-2-PBS targets N-cadherin as part of a matched antibody pair:

MP50439-1: 66219-2-PBS capture and 68532-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms CDHN, CDH2, CD325, Cadherin-2, Cadherin 2
Calculated Molecular Weight100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19022-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).