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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 68642-1-Ig (same clone as 68642-1-PBS)

    WB analysis using 68642-1-Ig (same clone as 68642-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 68642-1-Ig (same clone as 68642-1-PBS)

    WB analysis of LNCaP using 68642-1-Ig (same clone as 68642-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51262-1

    Cytometric bead array standard curve of MP51262-1

    Cytometric bead array standard curve of MP51262-1, COG1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68642-1-PBS. Detection antibody: 68642-2-PBS. Standard:Ag33924. Range: 3.125-200 ng/mL.

COG1 Monoclonal antibody, PBS Only (Capture)

COG1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68642-1-PBS
CloneNo. 2D8D11

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

68642-1-PBS targets COG1 as part of a matched antibody pair:

MP51262-1: 68642-1-PBS capture and 68642-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rabbit
Immunogen COG1 fusion protein Ag33924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name component of oligomeric golgi complex 1
Synonyms LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11
Calculated Molecular Weight 109 kDa
Observed Molecular Weight100-109 kDa
GenBank Accession NumberBC021985
Gene Symbol COG1
Gene ID (NCBI) 9382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WTW3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).