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golgin 97 Monoclonal antibody, PBS Only (Detector)

golgin 97 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5E4

Cat no : 68648-1-PBS

Print datasheet

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 2A5E4

验证数据展示

  • WB analysis using 68648-1-Ig (same clone as 68648-1-PBS)

    WB analysis using 68648-1-Ig (same clone as 68648-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68648-1-Ig (golgin 97 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68648-1-Ig (same clone as 68648-1-PBS)

    IF Staining of MCF-7 using 68648-1-Ig (same clone as 68648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:0 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68648-1-Ig (same clone as 68648-1-PBS)

    IF Staining of HeLa using 68648-1-Ig (same clone as 68648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:0 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50871-1

    Cytometric bead array standard curve of MP50871-1

    Cytometric bead array standard curve of MP50871-1, GOLGA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68648-2-PBS. Detection antibody: 68648-1-PBS. Standard:Ag33790. Range: 0.195-100 ng/mL.

  • Cytometric bead array standard curve of MP50871-3

    Cytometric bead array standard curve of MP50871-3

    Cytometric bead array standard curve of MP50871-3, GOLGA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68648-3-PBS. Detection antibody: 68648-1-PBS. Standard:Ag33790. Range: 1.563-100 ng/mL.

产品信息

68648-1-PBS targets golgin 97 as part of a matched antibody pair:

MP50871-1: 68648-2-PBS capture and 68648-1-PBS detection (validated in Cytometric bead array)

MP50871-3: 68648-3-PBS capture and 68648-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen golgin 97 fusion protein Ag33790 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 1
Synonyms GOLGA1, Golgin-97, Golgin subfamily A member 1, 2A5E4
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92805
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.