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Cleaved Caspase 3; p17 Monoclonal antibody

Cleaved Caspase 3; p17 Monoclonal Antibody for WB, IF, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, IF, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2F7B8

Cat No : 68773-1-Ig

Print datasheet

Synonyms

Caspase3, CASP3, CASP-3, Caspase 3, 2F7B8, CASP 3, Caspase-3, Caspase-3 subunit p12, Caspase-3 subunit p17, CPP-32, EC:3.4.22.56, Protein Yama, SCA-1, SREBP cleavage activity 1

验证数据展示

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 68773-1-Ig

    FC experiment of Jurkat using 68773-1-Ig

    1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.1 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

  • FC experiment of Jurkat using 68773-1-Ig

    FC experiment of Jurkat using 68773-1-Ig

    1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 68773-1-Ig

    IF Staining of Jurkat using 68773-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated Jurkat cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ).

  • IF Staining of HSC-T6 using 68773-1-Ig

    IF Staining of HSC-T6 using 68773-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

经过测试的应用

Positive WB detected inJurkat cells, A2780 cells, HepG2 cells, HSC-T6 cells, Ramos cells
Positive IF detected in1 μM Staurosporine (1/3/6 hour) treated Jurkat cells, 1 μM Staurosporine (1/3/6 hour) treated HSC-T6 cells
Positive FC detected in1 μM Staurosporine (3 hours) treated Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68773-1-Ig targets Cleaved Caspase 3; p17 in WB, IF, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF, ELISA, FC (Intra) Application Description
Cited ApplicationsWB
Tested Reactivity Human, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms Caspase3, CASP3, CASP-3, Caspase 3, 2F7B8, CASP 3, Caspase-3, Caspase-3 subunit p12, Caspase-3 subunit p17, CPP-32, EC:3.4.22.56, Protein Yama, SCA-1, SREBP cleavage activity 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberNM_032991
Gene Symbol Caspase 3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

实验方案

Product Specific Protocols
WB protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
IF protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Nutr

Structural Characterization and Anti-breast Cancer Activity in vitro of a Novel Polysaccharide From Cymbopogon citratus

Authors - Yi Chen
View Article