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Cleaved Caspase 3; P17 Monoclonal antibody, PBS Only

Cleaved Caspase 3; P17 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F7B8

Cat No : 68773-1-PBS

Print datasheet

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

验证数据展示

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    IF Staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 1 μM Staurosporine (1/3/6 hour) treated Jurkat cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

产品信息

68773-1-PBS targets Cleaved Caspase 3; P17 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Calculated Molecular Weight 32 kDa
GenBank Accession NumberNM_032991
Gene Symbol CASP3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42574
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).