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验证数据展示

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • IF Staining of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

    IF Staining of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 50 μM Cisplatin (18 hours) treated BV-2 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.1 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • FC experiment of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

    FC experiment of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

    1x10^6 non-treated BV-2 cells (dash lines) and cisplatin treated BV-2 cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    IP experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    IP result of anti-Cleaved Caspase 3/P17/P19 (IP:68773-1-Ig, 4ug; Detection:68773-1-Ig 1:5000) with Staurosporine treated Jurkat cells lysate 720 ug. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

    IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cleaved Caspase 3 antibody included
  • IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

    IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cleaved Caspase 3 antibody included
  • IHC staining of human ovary cancer using 68773-1-Ig (same clone as 68773-1-PBS)

    IHC staining of human ovary cancer using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cleaved Caspase 3 antibody included
  • IHC staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    IHC staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cleaved Caspase 3 antibody included
  • IF Staining of mouse liver using 68773-1-Ig (same clone as 68773-1-PBS)

    IF Staining of mouse liver using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    IF Staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated Jurkat cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

  • IF Staining of HSC-T6 using 68773-1-Ig (same clone as 68773-1-PBS)

    IF Staining of HSC-T6 using 68773-1-Ig (same clone as 68773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

Cleaved Caspase 3/P17/P19 Monoclonal antibody, PBS Only

Cleaved Caspase 3/P17/P19 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 68773-1-PBS

产品说明书

CloneNo. 2F7B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

别名

CASP3, Caspase3, Caspase 3, CASP-3, CASP 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Cleaved Caspase 3 Ready-To-Use IHC Kit. Cleaved Caspase 3 primary antibody included.

产品信息

68773-1-PBS targets Cleaved Caspase 3/P17/P19 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 3, apoptosis-related cysteine peptidase
别名 CASP3, Caspase3, Caspase 3, CASP-3, CASP 3
计算分子量 32 kDa
观测分子量17 kDa, 19 kDa
GenBank蛋白编号NM_032991
基因名称 Caspase 3
Gene ID (NCBI) 836
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42574
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).